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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法同时测定珠子草中没食子酸、短叶苏木酚、柯里拉京、鞣花酸和芦丁的含量

Simultaneous determination of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin in Phyllanthus niruri. Linn. by HPLC

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (9):1641-1645
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立反相高效液相色谱法同时测定珠子草中没食子酸、短叶苏木酚、柯里拉京、鞣花酸和芦丁5个化学成分的含量,并对方法学进行考察。方法:采用菲罗门Gemini C18柱(250 mm×4.6 mm,5 μm);流动相:乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱(0~20 min,3% A→15% A;20~45 min,15% A→28% A;45~55 min,100% A;56~65 min,3% A);流速:1 mL·min-1;柱温:室温;检测波长:254 nm。结果:没食子酸、短叶苏木酚、柯里拉京、鞣花酸和芦丁的进样量分别在0.072~1.84 μg、0.172~4.32 μg、0.124~3.12 μg、0.076~1.92 μg、0.156~3.97 μg范围内与峰面积呈良好的线性关系。测定珠子草药材粉末5份,没食子酸、短叶苏木酚、柯里拉京、鞣花酸、芦丁平均含量分别为0.21%、0.53%、0.24%、0.41%、0.11%。结论:本方法经方法学验证可用于珠子草多成分质量评价方法。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish and validate the HPLC method for simultaneous determination of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin in Phyllanthus niruri.Linn.Method: Gemini C18 (250 mm×4.6 mm, 5μm)column was adopted with mobile phase consisting of acetonitrile (A)and 0.1% phosphoric acid (B)in gradient elution (0-20 min, 3%A→15%A;20-45 min, 15%A→28%A;45-55 min, 100%A;56-65 min, 3%A).The flow rate was 1 mL·min-1, the column was kept at room temperature, and the detection wavelength was 254 nm.Result: The linear ranger of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin were 0.072-1.84 μg, 0.172-4.32 μg, 0.124-3.12 μg, 0.076-1.92 μg and 0.156-3.97 μg, respectively.The average contents of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin in 5 samples were 0.21%, 0.53%, 0.24%, 0.41% and 0.11%.Conclusion: This method was proved to be suitable for multi-component determination of Phyllanthus niruri.Linn.

-----参考文献:---------------------------------------------------------------------------------------
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