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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法测定发酵液中非达霉素的含量

Determination of fidaxomicin in fermentation broth by HPLC

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (9):1496-1499
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立高效液相色谱法定量检测微生物发酵液中的非达霉素。方法:采用Agilent 5 TC-C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇-0.1%甲酸水为流动相,流速1.0 mL·min-1,检测波长228 nm,柱温40℃。结果:非达霉素质量浓度在6.59~79.08 μg·mL-1范围内与峰面积呈现良好的线性关系(r=0.999 9,n=7);平均回收率为98.6%(n=9,RSD=0.91%);精密度、重复性和稳定性良好,RSD均小于2%;3批发酵液中非达霉素的测定结果分别为59.4、52.5、53.6 μg·mL-1结论:本方法可用于非达霉素的菌株筛选以及发酵过程产物的控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for quantitative determination of fidaxomicin in fermentation broth. Methods: The separation was carried out on the Agilent 5 TC-C18 (250 mm×4.6 mm, 5 μm)column at the detection wavelength of 228 nm and the column temperature was set at 40℃. The mobile phase consisted of methanol-0.1% formic acid solution at a flow rate of 1.0 mL·min-1. Results: The mass concentration of fidaxomicin and peak area showed excellent linearity within the range of 6.59-79.08 μg·mL-1, and the correlation coefficient was 0.999 9 (n=7). The average recovery (n=9) was 98.6% (RSD=0.91%). The precision, reproducibility and stability were satisfactory with RSD of less than 2%. The contents of fidaxomicin in three batches of fermentation broths were 59.4, 52.5 and 53.6 μg·mL-1, respectively. Conclusion: The developed method is suitable for strain screening and fermentation control of fidaxomicin.

-----参考文献:---------------------------------------------------------------------------------------
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